Uncaria gambir, a well known Southeast Asia plant have been previously used as an alternative medicine for treatment such as diarrheal, sore throat and spongy gums.Due to its useful properties, in FIRM HOLD HAIR SPRAY this study we have analysed the total phenol, condensed tannin, flavonoid content and antioxidant activity of Uncaria gambir in three different solvent extracts.Characterization and quantification analysis using Fourier Transform Infrared (FTIR) spectroscopy and reverse phase-high performance liquid chromatography (RP-HPLC) has confirmed that the major chemical constituents of Uncaria gambir are mainly catechins.It was revealed that the ethyl acetate gambir extract 645 gives the highest catechin content and antioxidant activity compared with other solvent extracts.